Parameters

General Parameters

To create synthetic data, the following parameters are available:

size: spatial dimensions of the resulting time frame, i.e. size=500results in 500 x 500 pixel frames

frames: number of time frames

spotsize: size of the individual spots

numpoints: total number of spots

motion: factor to determine the speed of the Brownian motion. Larger values correspond to faster motion.

brightness: relative brightness of the spots

Also necessary is the definition of a point spread function to convolve (and eventually deconvolve) with. For psf parameters, see usage.

Noise Parameters

Optionally, the synthetic data can be artificially corrupted with either or both Gaussian and Poisson noise. The level of noise is controlled by two corresponding parameters g and p. Larger values correspond to higher levels of noise.